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Luminescence & Fluorescence Immunoassays
The PdX™ Palladium API Fluorescent Detection Kit is designed to allow rapid screening of methods for Pd removal from solutions of APIs in DMSO, DMF, NMP, Ethanol, dilute HCl and Toluene. Standards or diluted samples are pipetted into a microtiter plate along with
supplied Detection Reagent and supplied Sodium Borohydride reducing agent. After 30 minutes the intensity of the generated fluorescence is read at 525 nm in a microtiter plate reader.
The DetectX™ Glutathione kit is designed to quantitatively measure GSH and GSSG present in a variety of samples. Total glutathione is the sum of the oxidized form plus the GSH. A GSH standard is provided to generate a standard curve. The kit utilizes a proprietary non-fluorescent molecule, ThioStar™, which will covalently bind to the free thiol group on GSH to yield a highly fluorescent product. After mixing the sample or standard with ThioStar™ and incubating at room temperature for 15 minutes, the GSH-generated signal is read at 510 nm in a fluorescent plate reader with excitation at 390 nm. Addition of a reaction mixture that converts all the GSSG into free GSH which then reacts with the excess ThioStar™ during a second 15 minute
Microplate Multimode Reader
Luminescence, Fluorescence and Absorbance
The Modulus™ Microplate Multimode Reader series is skillfully designed for today’s life science laboratory. They combine the superior performance expected from single-mode instruments with the functionality of multiple modes. The Modulus™ readers have plug-and-play usability; they combine a color LCD touch screen with an onboard computer, which eliminates the connectivity hassles, cost, and space requirements of running an external computer. Once an assay is run, data can be transferred in Excel-compatible .csv format using the built-in USB drive and the USB stick that comes with the instrument.
The Modulus™ Microplate is a modular instrument that easily fits into most budgets. Purchase the technology or modes that you need now and add onto the system later as your needs expand. For example, the Modulus™ Microplate can be purchased as a luminometer, then fluorescence and/or absorbance modules can be purchased and added later. Installations take less than ten minutes and can be done right in your lab. There is no need to download and install new software. The Modulus™ Microplate instantly detects the newly installed module(s) and will automatically adjust screens, protocols, and options.
· Dual reporter Assays
· Steady/Flash Glow Luciferase Assays
· Cell Viability/ ATP Assays
· Kinetics Assays
· Quant-iT Assays
· Fluorescein Measurement
· Cytotoxicity Assays
· Protease Assays
· BCA/Bradford Protein Assays
· Protease Assays
· Peroxide Assays
Both single and dual injectors are available for the Modulus Microplate Luminometer. Each injector has a volume range of 25 – 200 µl in 5 µl increments. Installed injector systems are automatically recognized. Injectors are recommended for labs running experiments with flash-based luminescence applications or dual-reporter assays.
An optional software program is available for those labs that would prefer to operate the Modulus Microplate Reader through an external PC.
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