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Cell Immortalization Vectors
As primary cells reach senescence after a limited number of population doublings, researchers frequently need to re-establish fresh cultures from explanted tissue -- a tedious process which can also add significant variation from one preparation to another. In order to have consistent material throughout a research project, researchers need primary cells with an extended replicative capacity, or immortalized cells. The ideal immortalized cells are cells that are not only capable of extended proliferation, but also possess similar or identical genotype and phenotype to their parental tissue.
Several methods exist for immortalizing mammalian cells in culture conditions.
We offer a comprehensive cell immortalization product line that is comprised of plasmids, retroviral, lentiviral and adenoviral vectors for hTERT, p53 and RB, siRNAs, and SV40 T antigens. We also offer these genes incorporated into ready-to-use recombinant retroviruses, lentivirus, and adenoviruses. All these tools will make your cell immortalization project simpler and easier than ever.
Hemostasis & Thrombosis ELISA
Our AssayMax ELISA kits are high quality in-vitro quantitative assays for detecting Hemostasis and Thrombosis, among others. These kits are easy, straight forward and give excellent results.
The AssayMax Human PAI-1 ELISA kit is designed for detection of PAI-1 in human plasma, tissue extracts or cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures PAI-1 in 4 hours. A polyclonal antibody specific for PAI-1 has been pre-coated onto a microplate. PAI-1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for PAI-1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measuredFeatures
· High specificity and reproducibility
· Easy to perform; rapid results
· Diverse biological samples
· Low sample volume required
· No specialized instrumentation required
· Unique targets; no other assays available
More information on www.gentaur.com
ProtoGel forms an electrophoresis matrix that is ideal for the separation of proteins and polypeptides. ProtoGel is a stabilized, ready-to-use 30% (w/v) acrylamide/methylene bisacrylamide solution (37.5:1 ratio), manufactured from the highest quality materials, from which virtually all impurities have been removed. ProtoGel has zero acrylic acid content, eliminating the fixed charges that cause band streaking. Additionally, oxidation products such as aldehydes have been removed by a selective adsorption process. Aldehydes can attack proteins, altering the structure of the sample, and thus altering Rf values. With ProtoGel, you can trust that your results will be consistent one electrophoretic run to the next.
· 37.5:1 Acrylamide to Bisacrylamide Stabilized Solution
· Optimized for SDS-PAGE of Proteins (Laemmli gels)
· Consistently Crystal Clear Gels, Zero Fluorescence
· Stabilized for Long Shelf Life
HIV-1 p24 ELISA
These quantitative antigen capture immunoassays detect viral antigen in research specimens including cell culture, serum and plasma. They are useful in monitoring viral cultures, purification procedures and biochemical behavior of the virus. The HIV-1 p24 Extended Range Kit is used to extend the range of the HIV-1 p24 ELISA Kit to detect viral loads up to 4 ng/ml. This eliminates the necessity for time consuming serial dilutions. Our Monoclonal Antibodies offer tremendous advantages over polyclonal antibodies because of their characteristics of homogeneity and specificity of binding.
· Reactive against antigens from HIV-1, HTLV-1 and HTLV-II
· Available in 100 µg and 1mg sizes
· Multiple clones available for most antigen targets
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