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rHuIL-4   GENTAUR HEADQUARTERS  +32 1641 4407  +32 1650 9045 

 rHuIL-2-3MIU huIFNa-2a huIFNa-2b Mouse GM-CSF rHuIL-4 GMPhuIL-4-50µg RHUGMCSF-300µg RHUGM-CSF-50µg huG-CSF-300µg huIL-6 -50 µg rhug-csf 300µg rhug-csf_75µg rhuil-1b-100µg


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Recombinant Human Interleukin-4 (research grade)

 ( rHuIL-4)      Price: 199 Euro/ 50 µg

 Certificate of Analysis and Data Sheet

 Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.


Source: Escherichia Coli.


Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.


Formulation & packaging: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. 


Solubility: The lyophilized rHuIL-4 is very soluble in water and most aqueous buffers below and above the isoelectric point.


Stability: Lyophilized rHuIL-4 although stable at room temperature, should be stored desiccated below 0°C. Reconstituted rHuIL-4 is best stored refrigerated at 4°C.


Purity: Greater than 99.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Anion-exchange FPLC.

(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
(Limit of acceptance:ł98.0%. No more than 2% total impurities; no single impurity greater than 1%)


Amino Acid Composition: In total agreement with the expected amino acid composition of native human IL-4.


Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Lys-Cys-Asp, conforming to the sequence of native human IL-4.


Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.


Biological Activity: ProSpec’s rHuIL-4 is fully biologically active when compared to standard.

The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 13 x106 IU/mg.


Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rHuIL-4.


Protein content:  Protein quantitation was carried out by two independent methods:

 1. UV spectroscopy at 280 nm.

 2. Analysis by RP-HPLC, using a calibrated solution of IL-4 as a Reference Standard.


Usage: This material is offered by Gentaur BVBA for research applications.


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Last modified: 06/25/09