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The World's Easiest Conjugation Kits
Direct labeling of antibodies can offer significant benefits in techniques such as flow cytometry, ELISA, western blotting and immunohistochemistry. Without the problems of crossover and/or non-specific binding from secondary reagents it becomes far easier to obtain good quality data. A reduction in the number of incubation and wash steps also makes assays less tedious and saves time. Unfortunately, there has been no simple way of making directly labeled primary antibodies - until now.
Lightning-Link™ uses a one-step procedure which means that conjugates can be prepared with ease. Hands-on time is less than 30 seconds, irrespective of scale. Separation steps are not required. Recovery of antibody is close to 100%.
Despite its simplicity, Lightning-Link™ is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled manner, creating high quality stable conjugates.
· Very easy to use
· No separation steps
· Virtually no losses
· Little antibody needed
· Easy to scale up
· 30 seconds hands on
Check out the complete range on www.gentaur.com
Annexin V Apoptosis kits
We offer you high quality annexin V products for fast detection of apoptosis in living cells. Annexin V conjugates are specially engineered to produce enhanced fluorescence signals and photostability, which perform considerably better than other similar products in the market. Annexin V assay is so simple, takes only 10 minutes. Results can be analyzed by flow cytometry or fluorescence microscopy. Complete kits of 25, 100, 400 assays sizes are available
Graphs: Detection of Apoptosis with Annexin V-EGFP. Apoptosis was induced in Jurkat cells by compthecin (2 mM) for various times as indicated. Cells were collected and incubated with Annexin V-EGFP for 5 minutes according to the kits instructions. Results were analysed by flow cytometry.
Solutions for Research
Gentaur offers you uncomplicated quantitative colorimetric or fluorimetric assays for drugs discovery, pharmacology and determinations directly in complex biological samples, food, beverages and environmental monitoring. The range includes 30 QuantiChrom™ kits, quantitative chemical colorimetric and fluorimetric assays and 8 EnzyChrom™, their enzymatic colorimetric counterparts varying from Blood/Urine Chemistry to Oxidative stress and Metabolism research.
For the High-throughput screening of Phosphatase, Phospholipase and other enzyme inhibitors we offer you the Malachite Green Phosphate Assay kit, sufficient for 2,500 assays in 96 well plates!
· Very stable reagents
· Sensitive and accurate
· Fast and convenient
· Robust and amenable to HTS
· Simple and direct
Vitotox™ 10 kit
Genotoxicity and Cytotoxcity test
The GENTAUR Vitotox™ 10 Kit is a unique method for rapid and cost effective high-throughput genotoxicity and toxicity screening.
Two recombinant Salmonella typhimurium TA104 test strains are used in the assay to determine simultaneously both genotoxicity and cytotoxicity of the sample. The test is based on luciferase reporter gene system which activity is measured by light emission and is a function of the genotoxicity of the test compound. Normally, the luciferase reporter gene is strongly repressed, but in the presence of a DNA damaging genotoxic compound the initiation of a cascade of reactions known as the SOS response will take place leading to derepression of the very strong promoter controlling the luciferase gene.
· Very small amount of sample required
· High reproducibility and sensitivity
· Very good correlation with AMES test
· Rapid answers in only 3 hours
· Low labor costs; automatic reading of kinetic data
· No test organism maintenance required
One of the major benefits over the Ames test is that the entire DNA content of the cell functions as a target for the genotoxin to display its effect. In contrast, the Ames test monitors mutations only in genes related to histidine synthesis. This results in a significantly lower sample concentrations (up to 1000 fold) required for the Vitotox™ 10 Kit.
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